Joint analysis of IVD herniation and degeneration by rat caudal needle puncture model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Cunha, C (Autor) Outra; Lamas, S (Autor) Outra; Goncalves, RM (Autor) ICBAS; Barbosa, MA (Autor) Outra

Revista

Título: Journal of Orthopaedic Research

Vol. 35

Páginas: 258-268

ISSN: 0736-0266

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 28 Citações

Scopus - 29 Citações

Outras Informações

ID Authenticus: P-00M-GMK

DOI: 10.1002/jor.23114

Abstract (EN): Intervertebral disc (IVD) degeneration is responsible for various spine pathologies and present clinical treatments are insufficient. Concurrently, the mechanisms behind IVD degeneration are still not completely understood, so as to allow development of efficient tissue engineering approaches. A model of rat IVD degeneration directly coupled to herniation is here proposed in a pilot study. Disc injury is induced by needle puncture, using two different needles gauges: a low caliber 25-G needle and a high caliber 21-G needle. Histological, biochemical, and radiographic degeneration was evaluated at 2 and 6 weeks post-injury. We show that the larger caliber needle results in a more extended histological and radiographic degeneration within the IVD, compared to the smaller one. TUNEL quantification indicates also increased cell death in the 21-G group. Analyses of collagen type I (Picrosirius red staining), collagen type II (immunofluorescence), and GAG content (Blyscan assay) indicate that degeneration features spontaneously recover from 2 to 6 weeks, for both needle types. Moreover, we show the occurrence of hernia proportional to the needle gauge. The number of CD68+ macrophages present, as well as cell apoptosis within the herniated tissue are both proportional to hernia volume. Moreover, hernias formed after lesion tend to spontaneously diminish in volume after 6 weeks. Finally, MMP3 is increased in the hernia in the 21-G group at 2 weeks. This model, by uniquely combining IVD degeneration and IVD herniation in the same animal, may help to understand mechanisms behind IVD pathophysiology, such as hernia formation and spontaneous regression. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:258-268, 2017.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11