Characterization of antibiotic resistant enterococci isolated from untreated waters for human consumption in Portugal

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Ana S Macedo (Autor) Outra; Ana R Freitas (Autor) Outra; Cristina Abreu (Autor) Outra; Elisabete Machado (Autor) Outra; Luisa Peixe (Autor) FFUP; Joao C Sousa (Autor) Outra; Carla Novais (Autor) FFUP

Revista

Título: International Journal of Food Microbiology

Vol. 145 Nº 1

Páginas: 315-319

ISSN: 0168-1605

Editora: Elsevier

Indexação

ISI Web of Knowledge - 22 Citações

ISI Web of Science

Scopus - 24 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-002-VR3

DOI: 10.1016/j.ijfoodmicro.2010.11.024

Resumo (PT): Untreated drinking water is frequently overlooked as a source of antibiotic resistance in developed countries. To gain further insight on this topic, we isolated the indicator bacteria Enterococcus spp. from water samples collected in wells, fountains and natural springs supplying different communities across Portugal, and characterized their antibiotic resistance profile with both phenotypic and genetic approaches. We found various rates of resistance to seven antibiotic families. Over 50% of the isolates were resistant to at least ciprofloxacin, tetracyclines or quinupristin–dalfopristin and 57% were multidrug resistant to ≥ 3 antibiotics from different families. Multiple enterococcal species (E. faecalis, E. faecium, E. hirae, E. casseliflavus and other Enterococcus spp) from different water samples harbored genes encoding resistance to tetracyclines, erythromycin or gentamicin [tet(M)-46%, tet(L)-14%, tet(S)-5%, erm(B)-22%, aac(6´)-Ie-aph(2″)-12%] and putative virulence factors [gel-28%, asa1-16%]. The present study positions untreated drinking water within the spectrum of ecological niches that may be reservoirs of or vehicles for antibiotic resistant enterococci/genes. These findings are worthy of attention as spread of antibiotic resistant enterococci to humans and animals through water ingestion cannot be dismissed. <br> <br> Keywords Human drinking water; Antibiotic resistance; tet genes; erm(B); aac(6´)-Ie-aph(2″); Virulence <br> <a target="_blank" href="http://www.sciencedirect.com/science/article/pii/S0168160510006549"> Texto integral </a> <br>

Abstract (EN): Untreated drinking water is frequently overlooked as a source of antibiotic resistance in developed countries. To gain further insight on this topic, we isolated the indicator bacteria Enterococcus spp. from water samples collected in wells, fountains and natural springs supplying different communities across Portugal, and characterized their antibiotic resistance profile with both phenotypic and genetic approaches. We found various rates of resistance to seven antibiotic families. Over 50% of the isolates were resistant to at least ciprofloxacin, tetracyclines or quinupristin-dalfopristin and 57% were multidrug resistant to antibiotics from different families. Multiple enterococcal species (E. faecalis, E. faecium, E. hirae, E. casseliflavus and other Enterococcus spp) from different water samples harbored genes encoding resistance to tetracyclines, erythromycin or gentamicin [tet(M)-46%, tet(L)-14%, ter(S)-5%, erm(B)-22%, aac(6')-le-aph(2 '')-12%] and putative virulence factors [gel-28%, asa1-16%). The present study positions untreated drinking water within the spectrum of ecological niches that may be reservoirs of or vehicles for antibiotic resistant enterococci/genes. These findings are worthy of attention as spread of antibiotic resistant enterococci to humans and animals through water ingestion cannot be dismissed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5