Automatic miniaturized fluorometric flow system for chemical and toxicological control of glibenclamide

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

David S M Ribeiro (Autor) Outra; Joao A V Prior (Autor) FFUP; Christian J M Taveira (Autor) Outra; Jose M A F S Mendes (Autor) Outra; Joao L M Santos (Autor) FFUP

Revista

Título: Talanta

Vol. 84

Páginas: 1329-1335

ISSN: 0039-9140

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

ISI Web of Science

Scopus - 6 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química > Química analítica

Outras Informações

ID Authenticus: P-002-QF8

DOI: 10.1016/j.talanta.2011.02.044

Resumo (PT): In this work, and for the first time, it was developed an automatic and fast screening miniaturized flow system for the toxicological control of glibenclamide in beverages, with application in forensic laboratory investigations, and also, for the chemical control of commercially available pharmaceutical formulations. The automatic system exploited the multipumping flow (MPFS) concept and allowed the implementation of a new glibenclamide determination method based on the fluorometric monitoring of the drug in acidic medium (λex = 301 nm; λem = 404 nm), in the presence of an anionic surfactant (SDS), promoting an organized micellar medium to enhance the fluorometric measurements. The developed approach assured good recoveries in the analysis of five spiked alcoholic beverages. Additionally, a good agreement was verified when comparing the results obtained in the determination of glibenclamide in five commercial pharmaceutical formulations by the proposed method and by the pharmacopoeia reference procedure. <br> <br> Keywords: Glibenclamide; Multipumping; Anti-diabetic; Fast screening; Drug-facilitated crime; Fluorescence <br> <a target="_blank" href="http://www.sciencedirect.com/science/article/pii/S0039914011001974"> Texto integral </a> <br> <br>

Abstract (EN): In this work, and for the first time, it was developed an automatic and fast screening miniaturized flow system for the toxicological control of glibenclamide in beverages, with application in forensic laboratory investigations, and also, for the chemical control of commercially available pharmaceutical formulations. The automatic system exploited the multipumping flow (MPFS) concept and allowed the implementation of a new glibenclamide determination method based on the fluorometric monitoring of the drug in acidic medium (lambda(ex) = 301 nm; lambda(em) = 404 nm), in the presence of an anionic surfactant (SDS), promoting an organized micellar medium to enhance the fluorometric measurements. The developed approach assured good recoveries in the analysis of five spiked alcoholic beverages. Additionally, a good agreement was verified when comparing the results obtained in the determination of glibenclamide in five commercial pharmaceutical formulations by the proposed method and by the pharmacopoeia reference procedure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7