A reagent-free method based on a photo-induced fluorimetry in a sequential injection system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Marieta L C Passos (Autor) Outra; Lucia L M F S Saraiva (Autor) FFUP; Joao L M Santos (Autor) FFUP; Salette Reis (Autor) FFUP; Marlene Lucio (Autor) FEUP; Jose L F C Lima (Autor) FFUP

Revista

Título: Talanta

Vol. 84

Páginas: 1309-1313

ISSN: 0039-9140

Editora: Elsevier

Indexação

ISI Web of Knowledge - 5 Citações

ISI Web of Science

Scopus - 4 Citações

Classificação Científica

CORDIS: Ciências Físicas > Química > Química analítica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-002-QF6

DOI: 10.1016/j.talanta.2011.02.037

Resumo (PT): According to the current demands of environmentally friendly analytical chemistry and with a view to achieving lower reagent consumption with improved analytical performance, an automatic methodology composed of a photoreactor and fluorimetric detection (λexc = 287 nm, λem = 378 nm) was developed. To this end, a sequential injection analysis (SIA) system was developed for indomethacin determination using ultra-violet (UV) light which promotes an increase in the fluorescence of indomethacin. This increase in sensitivity makes it possible to apply this methodology to a dissolution test and to determine indomethacin in pharmaceutical formulations. The calibration graph for indomethacin was linear between 4.10 × 10−6 and 9.00 × 10−5 mol L−1and the detection limit was 1.23 × 10−6 mol L−1. The method was proven to be reproducible with a R.S.D. < 5% and sampling rate of approximately 20 per hour. The potential effect of several compounds commonly used as excipients on analytical signals was studied and no interfering effect was observed. Statistical evaluation at the 95% confidence level showed good agreement between the results obtained for the pharmaceutical samples with both the SIA system and comparison batch procedures. <br> <br> Keywords: Sequential injection analysis; Indomethacin; Photochemically induced fluorescence; Green analytical chemistry <br> <a target="_blank" href="http://www.sciencedirect.com/science/article/pii/S0039914011001901 "> Texto integral </a> <br> <br>

Abstract (EN): According to the current demands of environmentally friendly analytical chemistry and with a view to achieving lower reagent consumption with improved analytical performance, an automatic methodology composed of a photoreactor and fluorimetric detection (lambda(exc) = 287 nm, lambda(em), = 378 nm) was developed. To this end, a sequential injection analysis (SIA) system was developed for indomethacin determination using ultra-violet (UV) light which promotes an increase in the fluorescence of indomethacin. This increase in sensitivity makes it possible to apply this methodology to a dissolution test and to determine indomethacin in pharmaceutical formulations. The calibration graph for indomethacin was linear between 4.10 x 10(-6) and 9.00 x 10(-5) mol L(-1) and the detection limit was 1.23 x 10(-6) mol L(-1). The method was proven to be reproducible with a R.S.D. <5% and sampling rate of approximately 20 per hour. The potential effect of several compounds commonly used as excipients on analytical signals was studied and no interfering effect was observed. Statistical evaluation at the 95% confidence level showed good agreement between the results obtained for the pharmaceutical samples with both the SIA system and comparison batch procedures.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5