Effects induced by the nodulation with Bradyrhizobium japonicum on Glycine max (soybean) metabolism and antioxidant potential

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Carla Couto (Autor) Outra; Luis R Silva (Autor) Outra; Patricia Valentao (Autor) FFUP; Encarna Velazquez (Autor) Outra; Alvaro Peix (Autor) Outra; Paula B Andrade (Autor) FFUP

Revista

Título: Food Chemistry

Vol. 127 Nº 4

Páginas: 1487-1495

ISSN: 0308-8146

Editora: Elsevier

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 25 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

CORDIS: Ciências da Saúde > Ciências farmacológicas > Farmacognosia

Outras Informações

ID Authenticus: P-002-NR3

DOI: 10.1016/j.foodchem.2011.01.135

Resumo (PT): This study was conducted in order to assess the effect of the inoculation of Glycine max (soybean) with Bradyrhizobium japonicum on the metabolite profile and antioxidant potential of its aerial parts. Extracts containing phenolic compounds and organic acids were analysed by HPLC–DAD and HPLC–UV, respectively. Volatiles profile was determined by HS-SPME/GC–IT-MS. The antioxidant activity of acidic and methanolic extracts was assessed against DPPH. Nodulation caused by B. japonicum led to an increase in the contents of phenolic compounds and organic acids. The same was observed for volatile compounds, with compounds like linalyl acetate, menthyl acetate and α-farnesene being detected only in inoculated plants. The phenolic extracts showed stronger antioxidant capacity than the organic acid extracts. In addition, extracts from plants nodulated with B. japonicum exhibited significantly higher antioxidant activity than control samples. These findings suggest that the inoculation with nodulating B. japonicum strains may be employed to manipulate the content of interesting metabolites in G. max aerial parts. <br> <br> Keywords: Glycine max; Bradyrhizobium japonicum; Volatile compounds; Organic acids; Phenolic compounds; Antioxidant capacity <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6R-523V3K6-3&_user=2460038&_coverDate=08%2F15%2F2011&_rdoc=11&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235037%232011%23998729995%233044776%23FLA%23display%23Volume)&_cdi=5037&_sort=d&_docanchor=&_ct=75&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=e0f92208bccef1d459af8d165974f547&searchtype=a "> Texto integral </a> <br> <br>

Abstract (EN): This study was conducted in order to assess the effect of the inoculation of Glycine max (soybean) with Bradyrhizobium japonicum on the metabolite profile and antioxidant potential of its aerial parts. Extracts containing phenolic compounds and organic acids were analysed by HPLC-DAD and HPLC-UV, respectively. Volatiles profile was determined by HS-SPME/GC-IT-MS. The antioxidant activity of acidic and methanolic extracts was assessed against DPPH. Nodulation caused by B. japonicum led to an increase in the contents of phenolic compounds and organic acids. The same was observed for volatile compounds, with compounds like linalyl acetate, menthyl acetate and alpha-farnesene being detected only in inoculated plants. The phenolic extracts showed stronger antioxidant capacity than the organic acid extracts. In addition, extracts from plants nodulated with B. japonicum exhibited significantly higher antioxidant activity than control samples. These findings suggest that the inoculation with nodulating B. japonicum strains may be employed to manipulate the content of interesting metabolites in G. max aerial parts.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9