Sample introduction in multi-syringe flow injection systems: comparison between time-based and volume-based strategies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Segundo, MA (Autor) REIT; Oliveira, HM (Autor) Outra; Lima, JLFC (Autor) FFUP; Almeida, MIGS (Autor) Outra; Rangel, AOSS (Autor) Outra

Revista

Título: Analytica Chimica Acta

Vol. 537

Páginas: 207-214

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 15 Citações

ISI Web of Science

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-3S2

DOI: 10.1016/j.aca.2005.01.009

Resumo (PT): In multi-syringe flow injection analysis (MSFIA), devices as selection, injection or commutation valves must be incorporated to the manifold to provide access to sample and standard solutions. Therefore, the definition of sample amount can be either volume or time-based. In the present work, four configurations for sample introduction (two for each approach) were tested in order to establish if the different strategies affect the analytical signal in MSFIA systems. The mean absorbance value from ten consecutive injections of a bromothymol blue solution obtained for the time-based strategy was lower than that provided by the volume-based approach as the exact volume delivered by each configuration was different from the “theoretical” volume. For time-based configurations, the exact volume delivered is 2–5% lower than the theoretical value while for volume-based configurations, the volume delivered was between 6 and 46% larger than the theoretical volume. Moreover, for time-based sampling, the order of steps in the analytical cycle was of utmost importance since any alteration in the flow direction affected the volume delivered in the subsequent step in the analytical cycle. The influence of the two sampling approaches was also evaluated in the MSFIA systems for the spectrophotometric determination of phenolic compounds and the potentiometric determination of chloride. There was no evidence that the use of either volume or time-based sampling would improve the analytical features of these determinations when real samples were tested. <br> <br> Keywords: Multi-syringe; Flow injection; Sampling strategy; Time-based sampling; Volume-based sampling <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4FD0N2M-J&_user=2460038&_coverDate=04%2F29%2F2005&_rdoc=26&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235216%232005%23994629998%23592300%23FLA%23display%23Volume)&_cdi=5216&_sort=d&_docanchor=&_ct=52&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=08725ccfc6150364d6ccbcd34ca2f556">Texto integral </a> <br> <br>

Abstract (EN): In multi-syringe flow injection analysis (MSFIA), devices as selection, injection or commutation valves must be incorporated to the manifold to provide access to sample and standard solutions. Therefore, the definition of sample amount can be either volume or time-based. In the present work, four configurations for sample introduction (two for each approach) were tested in order to establish if the different strategies affect the analytical signal in MSFIA systems. The mean absorbance value from ten consecutive injections of a bromothymol blue solution obtained for the time-based strategy was lower than that provided by the volume-based approach as the exact volume delivered by each configuration was different from the "theoretical" volume. For time-based configurations, the exact volume delivered is 2-5% lower than the theoretical value while for volume-based configurations, the volume delivered was between 6 and 46% larger than the theoretical volume. Moreover, for time-based sampling, the order of steps in the analytical cycle was of utmost importance since any alteration in the flow direction affected the volume delivered in the subsequent step in the analytical cycle. The influence of the two sampling approaches was also evaluated in the MSFIA systems for the spectrophotometric determination of phenolic compounds and the potentiometric determination of chloride. There was no evidence that the use of either volume or time-based sampling would improve the analytical features of these determinations when real samples were tested.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8