Simultaneous chemiluminometric determination of levodopa and benserazide in a multi-pumping flow system with multivariate calibration

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Karine L Marques (Autor) Outra; Joao L M Santos (Autor) FFUP; Joao A Lopes (Autor) Outra; Jose L F C Lima (Autor) FFUP

Revista

Título: Analytical Sciences

Vol. 24 Nº 10

Páginas: 985-991

ISSN: 0910-6340

Editora: Japan Society for Analytical Chemistry

Indexação

ISI Web of Knowledge - 16 Citações

ISI Web of Science

Scopus - 16 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-003-X45

DOI: 10.2116/analsci.24.985

Resumo (PT): An automated multi-pumping flow system is proposed for the simultaneous chemiluminometric determination of benserazide and levodopa using multivariate calibration methods. The developed methodology is based on chemiluminescence (CL) emission generated by the reaction of benserazide with luminol, and on a concurrent inhibiting effect of levodopa on this reaction. A multi-pumping flow system comprising multiple solenoid micro-pumps as the only active components was developed to implement a stopped-flow approach for signal acquisition and processing. Artificial neural networks were used to establish a relationship between the CL emission profile and the concentration of both drugs. The concentration values used to establish the experimental calibration samples were varied between 5 and 30 mg l-1 for levodopa and between 2.5 and 20 mg l-1 for benserazide. The proposed method was successfully applied to the simultaneous determination of levodopa and benserazide in pharmaceutical formulations combining both drugs. <br> <br> <a target="_blank" href="http://www.jstage.jst.go.jp/article/analsci/24/8/24_985/_article"> Texto integral</a> <br> <br>

Abstract (EN): An automated multi-pumping flow system is proposed for the simultaneous chemiluminometric determination of benserazide and levodopa using multivariate calibration methods. The developed methodology is based on chemiluminescence (CL) emission generated by the reaction of benserazide with luminol, and on a concurrent inhibiting effect of levodopa on this reaction. A multi-pumping flow system comprising multiple solenoid micro-pumps as the only active components was developed to implement a stopped-flow approach for signal acquisition and processing. Artificial neural networks were used to establish a relationship between the CL emission profile and the concentration of both drugs. The concentration values used to establish the experimental calibration samples were varied between 5 and 30 mg l(-1) for levodopa and between 2.5 and 20 mg l(-1) for benserazide. The proposed method was successfully applied to the simultaneous determination of levodopa and benserazide in pharmaceutical formulations combining both drugs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7