Specific modulation of apoptosis and Bcl-xL phosphorylation in yeast by distinct mammalian protein kinase C isoforms

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Lucilia Saraiva (Autor) FFUP; Rui D Silva (Autor) Outra; Gil Pereira (Autor) Outra; Jorge Goncalves (Autor) FFUP; Manuela Corte Real (Autor) Outra

Revista

Título: Journal of Cell Science

Vol. 119 Nº 15

Páginas: 3171-3181

ISSN: 0021-9533

Editora: The Company of Biologists Ltd.

Indexação

ISI Web of Knowledge - 30 Citações

ISI Web of Science

Scopus - 33 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-004-J66

DOI: 10.1242/jcs.03033

Resumo (PT): Mammalian protein kinase C (PKC) isoforms have been subject of particular attention because of their ability to modulate apoptotic proteins. However, the roles played by each PKC isoform in apoptosis are still unclear. Here, expression of individual mammalian PKC isoforms in Saccharomyces cerevisiae is used as a new approach to study the role of each isoform in apoptosis. The four isoforms tested, excepting PKC-d, stimulate S. cerevisiae acetic-acid-induced apoptosis essentially through a mitochondrial ROS-dependent pathway. However, their co-expression with Bcl-xL reveals a PKC-isoform-dependent modulation of Bcl-xL anti-apoptotic activity. A yeast pathway homologue to the mammalian SAPK/JNK is responsible for acetic-acid-induced Bcl-xL phosphorylation that is differently modulated by PKC isoforms. The data obtained suggest conservation of an ancient mechanism of apoptosis regulation in yeast and mammals and offer new insights into mammalian apoptosis modulation by PKC isoforms. <br> <br> <a target="_blank" href="http://ejournals.ebsco.com/Article.asp?ContributionID=9519059"> Texto integral</a> <br> <br>

Abstract (EN): Mammalian protein kinase C (PKC) isoforms have been subject of particular attention because of their ability to modulate apoptotic proteins. However, the roles played by each PKC isoform in apoptosis are still unclear. Here, expression of individual mammalian PKC isoforms in Saccharomyces cerevisiae is used as a new approach to study the role of each isoform in apoptosis. The four isoforms tested, excepting PKC-delta, stimulate S. cerevisiae acetic-acid-induced apoptosis essentially through a mitochondrial ROS-dependent pathway. However, their coexpression with Bcl-xL reveals a PKC-isoform-dependent modulation of Bcl-xL anti-apoptotic activity. A yeast pathway homologue to the mammalian SAPK/JNK is responsible for acetic-acid-induced Bcl-xL phosphorylation that is differently modulated by PKC isoforms. The data obtained suggest conservation of an ancient mechanism of apoptosis regulation in yeast and mammals and offer new insights into mammalian apoptosis modulation by PKC isoforms.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11