A pulsed sequential injection analysis flow system for the fluorimetric determination of indomethacin in pharmaceutical preparations

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Pinto, PCAG (Autor) Outra; Saraiva, MLMFS (Autor) FFUP; Santos, JLM (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: Analytica Chimica Acta

Vol. 539 Nº 1

Páginas: 173-179

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 30 Citações

ISI Web of Science

Scopus - 35 Citações

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-3CJ

DOI: 10.1016/j.aca.2005.03.037

Resumo (PT): This works reports the development of a pulsed flow sequential injection analysis (SIA) system based on the utilisation of pulse generating solenoid micro-pumps, which replace the conventional solutions propelling units commonly used in SIA systems. The influence on sample/reagent zones interpenetration and reaction zone formation of the reproducible pulsed flow produced by micro-pumps operation, which is characterised by a chaotic solutions movement, was comparatively evaluated with the typical laminar flow conditions of conventional SIA systems. The enhanced sample/reagent mixing provided by the controlled pulsed flow was favourably applied in the implementation of a SIA methodology for the fluorimetric determination of indomethacin in pharmaceutical preparations upon alkaline hydrolysis in micellar medium. Linear calibration plots were obtained for indomethacin concentrations up to 10−5 mol l−1 with a 3σ detection limit of about 1.6 × 10−8 mol l−1. The obtained results complied with those furnished by the reference procedure (RD ≤ 2.3%) and exhibited good reproducibility (RSD < 1.2%, n = 15). Sampling rate was about 30 samples per hour. <br> <br> Keywords: Pulsed flow; SIA; Solenoid micro-pump; Indomethacin; Micellar medium; Fluorimetry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4FXG2W5-2&_user=2460038&_coverDate=05%2F10%2F2005&_rdoc=26&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235216%232005%23994609998%23594416%23FLA%23display%23Volume)&_cdi=5216&_sort=d&_docanchor=&_ct=53&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=0d8686aac3a5c66c38231dba196c6f21"> Texto integral</a> <br> <br>

Abstract (EN): This works reports the development of a pulsed flow sequential injection analysis (SIA) system based on the utilisation of pulse generating solenoid micro-pumps, which replace the conventional solutions propelling units commonly used in SIA systems. The influence on sample/reagent zones interpenetration and reaction zone formation of the reproducible pulsed flow produced by micro-pumps operation, which is characterised by a chaotic solutions movement, was comparatively evaluated with the typical laminar flow conditions of conventional SIA systems. The enhanced sample/reagent mixing provided by the controlled pulsed flow was favourably applied in the implementation of a SIA methodology for the fluorimetric determination of indomethacin in pharmaceutical preparations upon alkaline hydrolysis in micellar medium. Linear calibration plots were obtained for indomethacin concentrations up to 10(-5) mol 1(-1) with a 3σ detection limit of about 1.6 x 10(-8) mol 1(-1). The obtained results complied with those furnished by the reference procedure (RD &LE; 2.3%) and exhibited good reproducibility (RSD < 1.2%, n = 15). Sampling rate was about 30 samples per hour.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7