Study of the potential toxicity of adrenaline to neurons, using the SH-SY5Y human cellular model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Costa, VM (Autor) Outra; Capela, JP (Autor) Outra; Bastos, ML (Autor) Outra; Fernando Remiao (Autor) FFUP; Varner, KJ (Autor) Outra; Duarte, JA (Autor) Outra; Felix Carvalho (Autor) FFUP

Revista

Título: Brazilian Journal of Pharmaceutical Sciences

Vol. 59

ISSN: 1984-8250

Editora: Universidade de São Paulo

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Y-FTP

DOI: 10.1590/s2175-97902023e20467

Abstract (EN): Prolonged overexposure to catecholamines causes toxicity, usually credited to continuous adrenoceptor stimulation, autoxidation, and the formation of reactive pro-oxidant species. Non-differentiated SH-SY5Y cells were used to study the possible contribution of oxidative stress in adrenaline (ADR)-induced neurotoxicity, as a model to predict the toxicity of this catecholamine to peripheral nerves. Cells were exposed to several concentrations of ADR (0.1, 0.25, 0.5 and 1mM) and two cytotoxicity assays [lactate dehydrogenase (LDH) release and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction] were performed at several time-points (24, 48, and 96h). The cytotoxicity of ADR was concentration -and time-dependent in both assays, since the lowest concentration tested (0.1mM) also caused significant cytotoxicity at 96h. N-acetyl-cysteine (1mM), a precursor of glutathione synthesis, prevented ADR-induced toxicity elicited by 0.5mM and 0.25mM ADR following a 96-h exposure, while the antioxidant Tiron (100 mu M) was non-protective. In conclusion, ADR led to mitochondrial distress and ultimately cell death in non-differentiated SH-SY5Y cells, possibly because of ADR oxidation products. The involvement of such processes in the catecholamine-induced peripheral neuropathy requires further analysis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10