Invasive pulmonary aspergillosis: current diagnostic methodologies and a new molecular approach

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2018-05-13

Autores

Cerqueira, L. (Autor) Outra; Sara Moura (Autor) Outra; Almeida, A (Autor) Outra

Revista

Título: European Journal of Clinical Microbiology and Infectious Diseases

Vol. 37

Páginas: 1393-1403

ISSN: 0934-9723

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-00N-YX5

DOI: 10.1007/s10096-018-3251-5

Abstract (EN): The fungus Aspergillus fumigatus is the main pathogenic agent responsible for invasive pulmonary aspergillosis. Immunocompromised patients are more likely to develop this pathology due to a decrease in the immune system's defense capacity. Despite of the low occurrence of invasive pulmonary aspergillosis, this pathology presents high rates of mortality, mostly due to late and unspecific diagnosis. Currently, the diagnostic methods used to detect this fungal infection are conventional mycological examination (direct microscopic examination, histological examination, and culture), imaging, non-culture-based tests for the detection of galactomannan, beta(1,3)-glucan and an extracellular glycoprotein, and molecular tests based on PCR. However, most of these methods do not detect the species A. fumigatus; they only allow the identification of genus Aspergillus. The development of more specific detection methods is of extreme importance. Fluorescent in situ hybridization-based molecular methods can be a good alternative to achieve this purpose. In this review, it is intended to point out that most of the methods used for the diagnosis of invasive pulmonary aspergillosis do not allow to detect the fungus at the species level and that fluorescence in situ hybridization-based molecular method will be a promising approach in the A. fumigatus detection.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11