Montelukast and cefoperazone act as antiquorum sensing and antibiofilm agents against Pseudomonas aeruginosa

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

Vieira, TF (Autor) Outra; Leitao, MM (Autor) Outra; Cerqueira, NMFSA (Autor) Outra; Sousa, SF (Autor) Outra; Anabela Borges (Autor) FEUP; Simoes, M (Autor) Outra; Simões M (Autor) FEUP

Revista

Título: JOURNAL OF APPLIED MICROBIOLOGY

Vol. 135

Páginas: 1-14

ISSN: 1364-5072

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Projetos Associados

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

InnovAntiBiofilm - Boosting Sustainable Innovation in Developing New Antibiotic Adjuvants to Control Biofilm Resistance

Outras Informações

ID Authenticus: P-010-CFP

DOI: 10.1093/jambio/lxae088

Abstract (EN): Aims Drug repurposing is an attractive strategy to control biofilm-related infectious diseases. In this study, two drugs (montelukast and cefoperazone) with well-established therapeutic applications were tested on Pseudomonas aeruginosa quorum sensing (QS) inhibition and biofilm control.Methods and results The activity of montelukast and cefoperazone was evaluated for Pqs signal inhibition, pyocyanin synthesis, and prevention and eradication of Ps. aeruginosa biofilms. Cefoperazone inhibited the Pqs system by hindering the production of the autoinducer molecules 2-heptyl-4-hydroxyquinoline (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (the Pseudomonas quinolone signal or PQS), corroborating in silico results. Pseudomonas aeruginosa pyocyanin production was reduced by 50%. The combination of the antibiotics cefoperazone and ciprofloxacin was synergistic for Ps. aeruginosa biofilm control. On the other hand, montelukast had no relevant effects on the inhibition of the Pqs system and against Ps. aeruginosa biofilm.Conclusion This study provides for the first time strong evidence that cefoperazone interacts with the Pqs system, hindering the formation of the autoinducer molecules HHQ and PQS, reducing Ps. aeruginosa pathogenicity and virulence. Cefoperazone demonstrated a potential to be used in combination with less effective antibiotics (e.g. ciprofloxacin) to potentiate the biofilm control action.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14