Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Melo, SA (Autor) Outra; Sugimoto, H (Autor) Outra; O'Connell, JT (Autor) Outra; Kato, N (Autor) Outra; Villanueva, A (Autor) Outra; Vidal, A (Autor) Outra; Qiu, L (Autor) Outra; Vitkin, E (Autor) Outra; Perelman, LT (Autor) Outra; Melo, CA (Autor) Outra; Lucci, A (Autor) Outra; Ivan, C (Autor) Outra; Calin, GA (Autor) Outra; Kalluri, R (Autor) Outra

Revista

Título: Cancer Cell

Vol. 26

Páginas: 707-721

ISSN: 1535-6108

Editora: Elsevier

Indexação

ISI Web of Knowledge - 992 Citações

Scopus - 1032 Citações

Outras Informações

ID Authenticus: P-00A-077

DOI: 10.1016/j.ccell.2014.09.005

Abstract (EN): Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosonnes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate nontumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15