Mitochondrial uncoupling proteins regulate the metabolic function of human Sertoli cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

Carrageta, DF (Autor) Outra; Freire-Brito, L (Autor) Outra; Guerra-Carvalho, B (Autor) Outra; Bernardino, RL (Autor) ICBAS; Monteiro, BS (Autor) Outra; barros, a (Autor) FMUP; Oliveira, PF (Autor) Outra; Monteiro, M (Autor) ICBAS; Alves, MG (Autor) Outra

Revista

Título: Reproduction

Vol. 167

ISSN: 1470-1626

Editora: BioScientifica Ltd.

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-GTB

DOI: 10.1530/rep-23-0229

Abstract (EN): Mitochondrial uncoupling proteins (UCPs) are mitochondrial channels responsible for the transport of protons and small molecular substrates across the inner mitochondrial membrane. Altered UCP expression or function is commonly associated with mitochondrial dysfunction and increased oxidative stress, which are both known causes of male infertility. However, UCP expression and function in the human testis remain to be characterized. This study aimed to assess the UCP homologs (UCP1-6) expression and function in primary cultures of human Sertoli cells (hSCs). We identified the mRNA expression of all UCP homologs (UCP1-6) and protein expression of UCP1, UCP2, and UCP3 in hSCs. UCP inhibition by genipin for 24 h decreased hSCs proliferation without causing cytotoxicity (n = 6). Surprisingly, the prolonged UCP inhibition for 24 h decreased mitochondrial membrane potential, oxygen consumption rate (OCR), and endogenous reactive oxygen species (ROS) production. The metabolism of hSCs was also affected as UCP inhibition shifted their metabolism toward an increased pyruvate consumption. Taken together, these findings demonstrate that UCPs play a role as regulators of the mitochondrial function in hSCs, emphasizing their potential as targets in the study of male (in)fertility.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14