Impact of Truncated O-glycans in Gastric-Cancer-Associated CD44v9 Detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Moreira, IB (Autor) Outra; Pinto, F (Autor) Outra; Gomes, C (Autor) Outra; Campos, D (Autor) Outra; Celso Reis (Autor) ICBAS

Revista

Título: Cells

Vol. 9

Página Final: 264

ISSN: 2073-4409

Editora: MDPI

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00R-Z1E

DOI: 10.3390/cells9020264

Abstract (EN): CD44 variant isoforms are often upregulated in cancer and associated with increased aggressive tumor phenotypes. The CD44v9 is one of the major protein splice variant isoforms expressed in human gastrointestinal cancer cells. Immunodetection of CD44 isoforms like CD44v9 in tumor tissue is almost exclusively performed by using specific monoclonal antibodies. However, the structural variability conferred by both the alternative splicing and CD44 protein glycosylation is disregarded. In the present work, we have evaluated the role of O-glycosylation using glycoengineered gastric cancer models in the detection of CD44v9 by monoclonal antibodies. We demonstrated, using different technical approaches, that the presence of immature O-glycan structures, such as Tn and STn, enhance CD44v9 protein detection. These findings can have significant implications in clinical applications mainly at the detection and targeting of this cancer-related CD44v9 isoform and highlight the utmost importance of considering glycan structures in cancer biomarker detection and in therapy targeting.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14