Molecular characterization of the MLL-SEPT6 fusion gene in acute myeloid leukemia: identification of novel fusion transcripts and cloning of genomic breakpoint junctions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

cerveira, n (Autor) Outra; Micci, F (Autor) Outra; Santos, J (Autor) Outra; Pinheiro, M (Autor) Outra; Correia, C (Autor) Outra; Lisboa, S (Autor) Outra; Bizarro, S (Autor) Outra; Norton, L (Autor) Outra; Glomstein, A (Autor) Outra; Asberg, AE (Autor) Outra; Heim, S (Autor) Outra; Manuel R Teixeira (Autor) ICBAS

Revista

Título: Haematologica

Vol. 93

Páginas: 1076-1080

ISSN: 0390-6078

Editora: FERRATA STORTI FOUNDATION

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-003-Y2M

DOI: 10.3324/haematol.12594

Abstract (EN): One of the MLL fusion partners in leukemia is the SEPT6 gene, which belongs to the evolutionarily conserved family of genes of septins. In this work we aimed to characterize at both the RNA and DNA levels three acute myeloid leukemias with cytogenetic evidence of a rearrangement between 11q23 and Xq24. Molecular analysis led to the identification of several MLL-SEPT6 fusion transcripts in all cases, including a novel MLL-SEPT6 rearrangement (MLL exon 6 fused with SEPT6 exon 2). Genomic DNA breakpoints were found inside or near Alu or LINE repeats in the MLL breakpoint cluster region, whereas the breakpoint junctions in the SEPT6 intron 1 mapped to the vicinity of GC-rich low-complexity repeats, Alu repeats, and a topoisomerase 11 consensus cleavage site. These data suggest that a non-homologous end-joining repair mechanism may be involved in the generation of MLL-SEPT6 rearrangements in acute myeloid leukemia.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5