Three Epigenetic Biomarkers, GDF15, TMEFF2, and VIM, Accurately Predict Bladder Cancer from DNA-Based Analyses of Urine Samples

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Costa, VL (Autor) Outra; henrique, rmf (Autor) ICBAS; Danielsen, SA (Autor) Outra; Duarte Pereira, S (Autor) Outra; Eknaes, M (Autor) Outra; Skotheim, RI (Autor) Outra; Rodrigues, A (Autor) Outra; Magalhaes, JS (Autor) Outra; Oliveira, J (Autor) Outra; Lothe, RA (Autor) Outra; Manuel R Teixeira (Autor) ICBAS; Carmen Jeronimo (Autor) ICBAS; Lind, GE (Autor) Outra

Revista

Título: Clinical Cancer Research

Vol. 16

Páginas: 5842-5851

ISSN: 1078-0432

Editora: American Association for Cancer Research

Indexação

ISI Web of Knowledge - 135 Citações

Scopus - 142 Citações

Outras Informações

ID Authenticus: P-003-0CT

DOI: 10.1158/1078-0432.ccr-10-1312

Abstract (EN): Purpose: To identify a panel of epigenetic biomarkers for accurate bladder cancer (BlCa) detection in urine sediments. Experimental Design: Gene expression microarray analysis of BlCa cell lines treated with 5-aza-2'deoxycytidine and trichostatin A as well as 26 tissue samples was used to identify a list of novel methylation candidates for BlCa. Methylation levels of candidate genes were quantified in 4 BlCa cell lines, 50 BlCa tissues, 20 normal bladder mucosas (NBM), and urine sediments from 51 BlCa patients and 20 healthy donors, 19 renal cancer patients, and 20 prostate cancer patients. Receiver operator characteristic curve analysis was used to assess the diagnostic performance of the gene panel. Results: GDF15, HSPA2, TMEFF2, and VIM were identified as epigenetic biomarkers for BlCa. The methylation levels were significantly higher in BlCa tissues than in NBM (P < 0.001) and the cancer specificity was retained in urine sediments (P < 0.001). A methylation panel comprising GDF15, TMEFF2, and VIM correctly identified BlCa tissues with 100% sensitivity and specificity. In urine samples, the panel achieved a sensitivity of 94% and specificity of 100% and an area under the curve of 0.975. The gene panel could discriminate BlCa from both healthy individuals and renal or prostate cancer patients (sensitivity, 94%; specificity, 90%). Conclusions: By usingagenome-wideapproach, we have identified a biomarker panel that allows for early and accurate noninvasive detection of BlCa using urine samples. Clin Cancer Res; 16(23); 5842-51. (C)2010 AACR.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10