Investigation of the Superoxide Anion-Triggered Chemiluminescence of Coelenterazine Analogs

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Erbiai, E (Autor) FCUP; Gonzalez-Berdullas, P (Autor) Outra; Joaquim C G E Esteves da Silva (Autor) FCUP; da Silva, LP (Autor) FCUP

Revista

Título: Applied Sciences

Vol. 13

Página Final: 6617

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Projetos Associados

ChemiTumorTher - Quimioluminescência Marinha como uma Terapia Anticancro de Amplo Espectro com uma Selectividade e Actividade Tumoral Melhorada

Outras Informações

ID Authenticus: P-00Y-HXH

DOI: 10.3390/app13116617

Abstract (EN): Reactive oxygen species (ROS), including superoxide anion, are involved in regulating various signaling pathways and are also responsible for oxidative stress. Sensing superoxide anion is of particular importance due to its biological significance. One potential approach is to use Coelenterazine as a chemiluminescent probe for the dynamic sensing of this ROS. In this study, we investigated the superoxide anion-triggered chemiluminescence of native Coelenterazine and two halogenated analogs and found that they showed a similar to 100-fold enhancement of light emission in aqueous solution, which was significantly reduced in methanol and nonexistent in aprotic solvents. In fact, Coelenterazine showed more intense light emission in aprotic solvents and, interestingly, although the light emission of the analogs seemed relatively unaffected by the solvents, their chemiluminescence was significantly quenched in water compared to methanol and, especially, to aprotic media. This suggests that the quenching effect observed for Coelenterazine is responsible for the differences in aqueous media, rather than an intrinsic enhanced emission by the analogs. In summary, we present Coelenterazine analogs that could serve as a basis for enhanced sensing of superoxide anion, providing information that could further our understanding of this chemiluminescent system.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11