Degradation Studies and Biological Behavior on an Artificial Cornea Material

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Livia Santos (Autor) Outra; Maria Pia Ferraz (Autor) FEUP; Yuki Shirosaki (Autor) Outra; Maria Ascensao Lopes (Autor) FEUP; Maria Helena Fernandes (Autor) FMDUP; Akiyoshi Osaka (Autor) Outra; Jose Domingos Santos (Autor) FEUP

Revista

Título: Investigative ophthalmology & visual science

Vol. 52 Nº 7

Páginas: 4274-4281

ISSN: 0146-0404

Indexação

ISI Web of Knowledge - 10 Citações

ISI Web of Science

Scopus - 17 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-002-QMK

DOI: 10.1167/iovs.10-6861

Abstract (EN): PURPOSE. Patients with dry eye syndrome, Stevens-Johnson syndrome, or recurrent transplant rejections are unsuitable to receive a keratoprosthesis. The present work aims at developing a highly biocompatible keratoprosthesis that could be successfully implanted in such patients. METHODS. Glass-reinforced hydroxyapatite (GRHA) was used to construct this new artificial cornea. To grant the device an adequate porosity, a porogen agent was added in the following percentages: 10, 30, and 50%. Samples were physicochemically analyzed in terms of density, porosity, roughness, degradation, and surface imaging. Biological relevance was assessed by cell culture, MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrasodium bromide) assays, and cell imaging. RESULTS. Samples B (30% porogen) and C (50% porogen) were found to be the most porous and also had the roughest topography. Degradation studies showed that under simulated physiologic conditions, no mass loss was found. Conversely, under acidic conditions, a significant mass loss was found. The biological performance of these samples was satisfactory when cultured with human fibroblasts. The MTT assay revealed that samples B and C had greater propensity to cell invasion and proliferation than that of the other tested materials. Cell imaging demonstrated that fibroblasts organized around the pore edges before colonizing it. CONCLUSIONS. A material with physicochemical and biological characteristics close to an ideal artificial cornea has been fabricated. The GRHA cornea containing 30% porogen is the most promising substitute material due to the biological performance, adequate porosity, and low degradation propensity. (Invest Ophthalmol Vis Sci. 2011;51:4274-4281) DOI:10.1167/iovs.10-6861

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8