Performance of immunohistochemical and molecular methods in detecting microsatellite instability in gastric cancer: a multicenter study

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Marques, DS (Autor) Outra; Gullo, I (Autor) Outra; Mascarenhas-Lemos, L (Autor) Outra; Silva, JR (Autor) Outra; do Nascimento, CN (Autor) Outra; Pontes, P (Autor) Outra; Pinho, L (Autor) Outra; Cirnes, L (Autor) Outra; Wen, XG (Autor) Outra; Cravo, M (Autor) Outra; Carneiro F (Autor) FMUP

Revista

Título: Pathobiology

Páginas: 1-1

ISSN: 1015-2008

Editora: Karger

Indexação

ISI Web of Knowledge - 0 Citações

Outras Informações

ID Authenticus: P-00Y-KM1

DOI: 10.1159/000530997

Abstract (EN): Introduction: Introduction: Microsatellite instability (MSI) is an important prognostic molecular biomarker for gastric cancer (GC). MSI status may be detected by immunohistochemistry (IHC) for mismatch repair (MMR) proteins and polymerase chain reaction (PCR). Idylla MSI assay has not been validated for GC but may prove to be a valid alternative.Methods: In a series of 140 GC cases, MSI status was evaluated by IHC for MLH1, PMS2, MSH2, and MSH6; gold-standard pentaplex PCR panel (PPP) (BAT-25, BAT-26, NR-21, NR-24, and NR-27); and Idylla. Statistical analysis was performed using SPSS 27.0.Results: PPP identified 102 microsatellite stable (MSS) cases and 38 MSI-high cases. Only three cases showed discordant results. Compared with PPP, the sensitivity was 100% for IHC and 94.7% for Idylla. Specificity was 99% for IHC and 100% for Idylla. MLH1 IHC alone showed sensitivity and specificity of 97.4% and 98.0%, respectively. IHC identified three indeterminate cases; all were MSS according to PPP and Idylla.Conclusion: IHC for MMR proteins represents an optimal screening tool for MSI status in GC. If resources are limited, isolated MLH1 evaluation may constitute a valuable option for preliminary screening. Idylla may help detect rare MSS cases with MMR-loss and define MSI status in indeterminate cases.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 25