O-glycan truncation enhances cancer-related functions of CD44 in gastric cancer

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Mereiter, S (Autor) Outra; Martins, AM (Autor) Outra; Gomes, C (Autor) Outra; Balmana, M (Autor) Outra; Macedo, JA (Autor) Outra; Polom, K (Autor) Outra; Roviello, F (Autor) Outra; Magalhaes, A (Autor) Outra; Celso Reis (Autor) ICBAS

Revista

Título: FEBS Letters

Vol. 593

Páginas: 1675-1689

ISSN: 0014-5793

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 18 Citações

Outras Informações

ID Authenticus: P-00Q-X8J

DOI: 10.1002/1873-3468.13432

Abstract (EN): CD44 isoforms are often upregulated in gastric cancer and have been associated with increased metastatic potential and poor survival. To evaluate the functional impact of O-glycan truncation on CD44 we have analysed glyco-engineered cancer cell models displaying shortened O-glycans. Here, we demonstrate that induction of aberrant O-glycan termination through various molecular mechanisms affects CD44 molecular features. We show that CD44 is a major carrier of truncated O-glycans and that this truncation is accompanied by an increased hyaluronan binding capacity and affects extracellular shedding. In addition, short O-glycans promoted the colocalization of CD44v6 with the receptor tyrosine kinase RON and concomitantly increased activation. Our in vitro findings were validated in gastric cancer clinical samples.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15