Secretion capacity limitations of the Sec pathway in Escherichia coli

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Mergulhao, FJM (Autor) Outra; Monteiro, GA (Autor) Outra

Revista

Título: Journal of Microbiology and Biotechnology

Vol. 14 Nº 1

Páginas: 128-133

ISSN: 1017-7825

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 14 Citações

ISI Web of Science

Scopus - 20 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Tecnológicas > Tecnologia > Biotecnologia

Outras Informações

ID Authenticus: P-000-BTD

Abstract (EN): The secretion capacity of two E. coli strains (JM109 and AF1000) was evaluated through the expression of two human proinsulin fusion proteins using the translocation signal sequence from Staphylococcal protein A (SpA). Although a 7 to 11-fold difference in the expression levels was attained by the use of different promoters (SpA and malK promoters) and copy-number vectors (700 and 50 copies per cell), the maximum translocation rates for all the systems were around 140,000 amino acids cell(-1) min(-1). Moreover, the secretion capacity was found to be independent of the size of the exiting peptide and its translational rate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6