The peptide secreted at the water to land transition in a model amphibian has antioxidant effects

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Barbosa, EA (Autor) Outra; Placido, A (Autor) Outra; Moreira, DC (Autor) Outra; Albuquerque, L (Autor) Outra; Dematei, A (Autor) Outra; Silva Carvalho, AE (Autor) Outra; Cabral, WF (Autor) Outra; Bao, SN (Autor) Outra; Saldanha Araujo, F (Autor) Outra; Kuckelhaus, SAS (Autor) Outra; Borges, TK (Autor) Outra; Portugal, CC (Autor) Outra; Socodato, R (Autor) Outra; Teixeira, C (Autor) Outra; Lima, FCDA (Autor) Outra; Batagin Neto, A (Autor) Outra; Sebben, A (Autor) Outra; Eaton, P (Autor) Outra; Paula Gomes (Autor) FCUP; Brand, GD (Autor) Outra; Relvas, JB (Autor) Outra; Kato, MJ (Autor) Outra; Leite, JRSA (Autor) Outra

Revista

Título: Proceedings of the Royal Society B: Biological Sciences

Vol. 288

ISSN: 0962-8452

Editora: The Royal Society

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00V-PXC

DOI: 10.1098/rspb.2021.1531

Abstract (EN): In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O-2 levels and UV exposure on land compared with aquatic environments.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10