Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Sousa-Cardoso, F (Autor) Outra; Rita Teixeira dos Santos (Autor) FEUP; Campos, AF (Autor) Outra; Lima, M (Autor) Outra; Luciana Gomes (Autor) FEUP; Soares, OSGP (Autor) Outra; Filipe Mergulhão (Autor) FEUP

Revista

Título: Nanomaterials

Vol. 13

Página Final: 381

ISSN: 2079-4991

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Projetos Associados

Healthy Waters - Healthy Waters - Identification, Elimination, Social Awareness and Education of Water Chemical and Biological Micropollutants with Health and Environmental Implications

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

NanoCAT - NanoCAT - Utilização de nanotubos de carbono modificados para combater infecções em catéteres urinários e stents

Outras Informações

ID Authenticus: P-00X-YPV

DOI: 10.3390/nano13030381

Abstract (EN): Due to its several economic and ecological consequences, biofouling is a widely recognized concern in the marine sector. The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pristine graphene nanoplatelets (GNP) on biofilm development through the representative marine bacteria Cobetia marina and to investigate the antibacterial mechanisms of action of this material. For this purpose, a flow cytometric analysis was performed and a GNP/polydimethylsiloxane (PDMS) surface containing 5 wt% GNP (G5/PDMS) was produced, characterized, and assessed regarding its biofilm mitigation potential over 42 days in controlled hydrodynamic conditions that mimic marine environments. Flow cytometry revealed membrane damage, greater metabolic activity, and endogenous reactive oxygen species (ROS) production by C. marina when exposed to GNP 5% (w/v) for 24 h. In addition, C. marina biofilms formed on G5/PDMS showed consistently lower cell count and thickness (up to 43% reductions) than PDMS. Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS. Overall, the GNP-based surface inhibited C. marina biofilm development, showing promising potential as a marine antifouling coating.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16