ICEs Are the Main Reservoirs of the Ciprofloxacin-ModifyingcrpPGene inPseudomonas aeruginosa

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Botelho, J (Autor) Outra; Grosso, F (Autor) Outra; Luisa Peixe (Autor) FFUP

Revista

Título: Genes

Vol. 11

ISSN: 2073-4425

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00S-K25

DOI: 10.3390/genes11080889

Abstract (EN): The ciprofloxacin-modifyingcrpPgene was recently identified in a plasmid isolated from aPseudomonas aeruginosaclinical isolate. Homologues of this gene were also identified inEscherichia coli,Klebsiella pneumoniaeandAcinetobacter baumannii. We set out to explore the mobile elements involved in the acquisition and spread of this gene in publicly available and complete genomes ofPseudomonasspp. AllPseudomonascomplete genomes were downloaded from NCBI's Refseq library and were inspected for the presence of thecrpPgene. The mobile elements carrying this gene were further characterized. ThecrpPgene was identified only inP. aeruginosa, in more than half of the complete chromosomes (61.9%,n= 133/215) belonging to 52 sequence types, of which the high-risk clone ST111 was the most frequent. We identified 136crpP-harboring integrative and conjugative elements (ICEs), with 93.4% belonging to the mating-pair formation G (MPFG) family. The ICEs were integrated at the end of a tRNA(Lys)gene and were all flanked by highly conserved 45-bp direct repeats. ThecrpP-carrying ICEs contain 26 core genes (2.2% of all 1193 genes found in all the ICEs together), which are present in 99% or more of thecrpP-harboring ICEs. The most frequently encoded traits on these ICEs include replication, transcription, intracellular trafficking and cell motility. Our work suggests that ICEs are the main vectors promoting the dissemination of the ciprofloxacin-modifyingcrpPgene inP. aeruginosa.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10