Effect of Prediabetes on Membrane Bicarbonate Transporters in Testis and Epididymis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Bernardino, RL (Autor) Outra; Martins, AD (Autor) Outra; Socorro, S (Autor) Outra; Alves, MG (Autor) Outra; Oliveira, PF (Autor) Outra

Revista

Título: Journal of Membrane Biology

Vol. 246

Páginas: 877-883

ISSN: 0022-2631

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 20 Citações

Outras Informações

ID Authenticus: P-008-HEZ

DOI: 10.1007/s00232-013-9601-4

Abstract (EN): The formation of competent spermatozoa is a complex event that depends on the establishment of adequate environments throughout the male reproductive tract. This includes the control of bicarbonate (HCO3 (-)) concentration, which plays an essential role in the maintenance of extracellular and intracellular pH (pH(i)) values. Diabetes mellitus alters pH(i) regulation in mammalian cells, mainly by altering the activity of ion transporters, particularly HCO3 (-)-dependent mechanisms. Yet, little is known about the effects of this pathology and its prodromal stage, prediabetes, on the membrane transport mechanisms of male reproductive tract cells. Herein, we analyzed protein and mRNA levels of the most relevant HCO3 (-) transporters of the SLC4 family [anion exchanger 2 (AE2), Na+-driven Cl-/HCO3 (-) exchanger (NDCBE), electrogenic Na+/HCO3 (-) cotransporter 1 (NBCe1), electroneutral Na+/HCO3 (-) cotransporter 1 (NBCn1)] in the testis and epididymis of a prediabetic animal model. Firstly, we identified the HCO3 (-) transporters of the SLC4 family, in both testicular and epididymal tissue. Secondly, although no alterations were detected in protein expression, mRNA levels of NBCe1, NBCn1 and NDCBE were significantly increased in the testis of prediabetic rats. On the other hand, in the epididymis, prediabetes caused an increase of AE2 and a decrease of NDCBE protein levels. These alterations may be translated into changes of HCO3 (-) transepithelial epididymal fluxes in vivo, which may represent a threat for sperm survival. Moreover, these results provide evidence of the molecular mechanism that may be responsible for the significant increase in abnormal sperm morphology already reported in prediabetic rats.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7