Solid lipid nanoparticles optimized by 2(2) factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Rigon, RB (Autor) Outra; Goncalez, ML (Autor) Outra; Severino, P (Autor) Outra; Alves, DA (Autor) Outra; Santana, MHA (Autor) Outra; Souto, EB (Autor) Outra; Chorilli, M (Autor) Outra

Revista

Título: Colloids and Surfaces B: Biointerfaces

Vol. 171

Páginas: 501-505

ISSN: 0927-7765

Editora: Elsevier

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 33 Citações

Outras Informações

ID Authenticus: P-00P-E0E

DOI: 10.1016/j.colsurfb.2018.07.065

Abstract (EN): The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5