Ocular Cell Lines and Genotoxicity Assessment

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2020

Autores

Souto, EB (Autor) Outra; Campos, JR (Autor) Outra; Da Ana, R (Autor) Outra; Martins Gomes, C (Autor) Outra; Silva, AM (Autor) Outra; Souto, SB (Autor) FMUP; Lucarini, M (Autor) Outra; Durazzo, A (Autor) Outra; Santini, A (Autor) Outra

Revista

Título: International Journal of Environmental Research and Public Health

Vol. 17 Nº 9

Página Final: 2046

ISSN: 1661-7827

Editora: MDPI

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Classificação Científica

CORDIS: Ciências Naturais

Outras Informações

ID Authenticus: P-00R-XMN

DOI: 10.3390/ijerph17062046

Abstract (EN): Genotoxicity screening tests aim to evaluate if and to what extent a compound in contact with the human body (e.g., a drug molecule, a compound from the environment) interacts with DNA. The comet assay is a sensitive method used to predict the risk of DNA damage in individual cells, as it quantifies the tape breaks, being the alkaline version (pH > 13) the most commonly used in the laboratory. Epithelial cells serve as biomatrices in genotoxicity assessments. As ca. 80% of solid cancers are of epithelial origin, the quantification of the DNA damage upon exposure of epithelial cells to a drug or drug formulation becomes relevant. Comet assays run in epithelial cells also have clinical applications in human biomonitoring, which assesses whether and to what extent is the human body exposed to environmental genotoxic compounds and how such exposure changes over time. Ocular mucosa is particularly exposed to environmental assaults. This review summarizes the published data on the genotoxicity assessment in estimating DNA damage in epithelial cells with a special focus on ocular cell lines. General comet assay procedures for ex vivo and in vivo epithelium samples are also described.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17