Adhesion of human leukocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: Effect of blood protein adsorption

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Judite N Barbosa (Autor) ICBAS; Martins, MCL (Autor) Outra; Sidonio C Freitas (Autor) Outra; Ines C Goncalves (Autor) FEUP; Artur P Aguas (Autor) ICBAS; Mario A Barbosa (Autor) FEUP

Revista

Título: Journal of Biomedical Materials Research - Part A

Vol. 93A Nº 1

Páginas: 12-19

ISSN: 1549-3296

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 13 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-003-7W5

DOI: 10.1002/jbm.a.32480

Abstract (EN): The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Self-assembled monolayers (SAMs) prepared with mixtures of methyl- and hydroxyl-terminated alkanethiols in different Percentages oil gold were used. The surfaces were pre-immersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre-immersion in plasma presented higher numbers of adherent leukocytes in the pure OH-terminated SAM, whereas methyl-terminated Surfaces accounted for the lowest number of adherent cells. We observed a general increase ill the number of adherent human leukocytes as the percentage of OH groups on the Surface of the SAMs increased for all the pre-immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre-immersion conditions used, and this observation is particularly relevant in the case of the methyl-terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre-immersion in protein solutions has a determinant effect in leukocyte adhesion. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 12-19, 2010

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8