The Osteogenic Assessment of Mineral Trioxide Aggregate- based Endodontic Sealers in an Organotypic Ex Vivo Bone Development Model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Gomes PS (Autor) FMDUP; Pinheiro, B (Autor) Outra; Colaco, B (Autor) Outra; Fernandes MH (Autor) FMDUP

Revista

Título: Journal of Endodontics

Vol. 47

Páginas: 1461-1466

ISSN: 0099-2399

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Pubmed / Medline

Outras Informações

ID Authenticus: P-00V-BXX

DOI: 10.1016/j.joen.2021.06.006

Abstract (EN): Introduction: Mineral trioxide aggregate (MTA)-based sealers are endodontic materials with widespread success in distinct clinical applications, potentially embracing direct contact with the bone tissue. Bone response to these materials has been traditionally addressed in vitro. Nonetheless, translational data are limited by the absence of native cell-to-cell and cell-to-matrix interactions that hinder the representativeness of the analysis. Ex vivo organotypic systems, relying on the culture of explanted biological tissues, preserve the cell/tissue composition, reproducing the spatial and organizational in situ complexity. This study was grounded on an innovative research approach, relying on the assessment of an ex vivo organotypic bone tissue culture system to address the osteogenic response to 3 distinct MTA-based sealers. Methods: Embryonic chick femurs were isolated and grown ex vivo for 11 days in the presence of MTA Plus (Avalon Biomed Inc, Bradenton, FL), ProRoot MTA (Dentsply Tulsa Dental, Hohnson City, Germany), Biodentine (Septodont, Saint Maurdes Fosses, France), or AH Plus (Dentsply Sirona, Konstanz, Germany); the latter was used as a control material. Femurs were characterized by histologic, histochemical, and histomorphometric analysis. Gene expression assessment of relevant osteogenic markers was conducted by quantitative polymerase chain reaction. Results: All MTA-based sealers presented an enhanced osteogenic performance compared with AH Plus. Histochemical and histomorphometric analyses support the increased activation of the osteogenic program by MTA-based sealers, with enhanced collagenous matrix deposition and tissue mineralization. Gene expression analysis supported the enhanced activation of the osteogenic program. Comparatively, ProRoot MTA induced the highest osteogenic functionality on the charac-terized femurs. Conclusions: MTA-based sealers enhanced the osteogenic activity within the assayed organotypic bone model, which was found to be a sensitive system for the assessment of osteogenic modulation mediated by endodontic sealers. (J Endod 2021;47:1461-1466.)

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6