Kinetic and Stability Study of the Peroxidase Inhibition in Ionic Liquids

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Aristides P. Carneiro (Autor) Outra; Oscar Rodríguez (Autor) FEUP; Fátima L. Mota (Autor) FEUP; Ana P. M. Tavares (Autor) FEUP; Eugénia A. Macedo (Autor) FEUP

Revista

Título: Industrial & Engineering Chemistry Research

Vol. 48

Páginas: 10810-10815

ISSN: 0888-5885

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 8 Citações

ISI Web of Science

Scopus - 9 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Outras ciências da engenharia e tecnologias

Outras Informações

ID Authenticus: P-003-DJV

DOI: 10.1021/ie9007612

Abstract (EN): The activity and stability of peroxidase in aqueous solutions of two ionic liquids (ILs) have been studied. The ILs selected were 1-ethyl-3-methylimidazolium 2-(2-methoxyethoxy) ethylsulfate, [emim][MDEGSO(4)], and 1-ethyl-3-methylimidazolium ethylsulfate, [emim][EtSO4]. Experiments were performed at room temperature using concentrations of ILs between 5 and 50% (v/v) and pH values in the range from 5 to 9. The initial activity of the enzyme in these ILs at optimized conditions (pH 7 and 5-10% IL) was similar or higher than that achieved with buffer solution. Kinetic studies showed that maximum reaction Velocity (V-max) decreased with increasing concentration of IL. The effect of [emim][EtSO4] concentration on the decrease of V-max was higher than that of [emim][MDEGSO(4)]. It was found that [emim][EtSO4] was a more potent inhibitor on peroxidase activity. The peroxidase studied was active in the ILs investigated, and the enzyme exhibited a higher stability in ILs at the optimized conditions. Kinetic studies showed that inhibition was a noncompetitive type in both ILs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6