Engineering Endochondral Bone: In Vitro Studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Serafim M. Oliveira (Autor) FEUP; Isabel F. Amaral (Autor) FEUP; Mário A. Barbosa (Autor) FEUP; Cristina C. Teixeira (Autor) Outra

Revista

Título: Tissue Engineering - Part A

Vol. 15 Nº 3

Páginas: 625-634

ISSN: 1937-3341

Editora: Mary Ann Liebert

Indexação

ISI Web of Knowledge - 33 Citações

ISI Web of Science

Scopus - 37 Citações

COMPENDEX

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-003-N3C

DOI: 10.1089/ten.tea.2008.0051

Abstract (EN): Chitosan scaffolds have been shown to possess biological and mechanical properties suitable for tissue engineering and clinical applications. In the present work, chitosan sponges were evaluated regarding their ability to support cartilage cell proliferation and maturation, which are the first steps in endochondral bone formation. Chitosan sponges were seeded with chondrocytes isolated from chicken embryo sterna. Chondrocyte/chitosan constructs were cultured for 20 days, and treated with retinoic acid (RA) to induce chondrocyte maturation and matrix synthesis. At different time points, samples were collected for microscopic, histological, biochemical, and mechanical analyses. Results show chondrocyte attachment, proliferation, and abundant matrix synthesis, completely obliterating the pores of the sponges. RA treatment caused chondrocyte hypertrophy, characterized by the presence of type X collagen in the extracellular matrix and increased alkaline phosphatase activity. In addition, hypertrophy markedly changed the mechanical properties of the chondrocyte/chitosan constructs. In conclusion, we have developed chitosan sponges with adequate pore structure and mechanical properties to serve as a support for hypertrophic chondrocytes. In parallel studies, we have evaluated the ability of this mature cartilage scaffold to induce endochondral ossification.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10