The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Cristina C Barrias (Autor) Outra; Cristina C L Martins (Autor) Outra; Graca Almeida Porada (Autor) Outra; Mario A Barbosa (Autor) FEUP; Pedro L Granja (Autor) FEUP

Revista

Título: Biomaterials

Vol. 30 Nº 36

Páginas: 307-316

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 81 Citações

ISI Web of Science

Scopus - 79 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-003-P9E

DOI: 10.1016/j.biomaterials.2008.09.048

Abstract (EN): The objective of this Study was to compare the biological effects of two key cell-adhesive proteins, fibronectin (FN) and vitronectin (VN), upon adsorption onto molecularly-designed model surfaces. Single-component and mixed self-assembled monolayers (SAMs) of alkanethiols on gold with OH and CH(3) terminal groups were prepared at 100%, 65%, 36% and 0% of OH at the surface, to generate a range of Surfaces with a simple chemistry and a wettability gradient. FN and VN were adsorbed under noncompetitive (single-protein solutions) and competitive (multi-protein solutions) conditions, and compared at different levels: adsorbed amount (radiolabelling), elution, functional presentation of cell-binding domains (ELISA), and role in mediating cell adhesion (antibody-based assay). The observed trends were related to mesenchymal stem cell response in terms of adhesion and overall cell morphology. Under non-competitive conditions, adsorption of both proteins increased with surface hydrophobicity. The presence of competitive proteins significantly decreased the adsorbed amounts, although both proteins were still detected in all SAMs. Adsorption of FN followed a trend similar to that of non-competitive conditions, while adsorption of VN was higher on 100%OH-SAMs. Concerning elution, retention of adsorbed VN was always higher than that of FN. For both proteins, functional presentation of cell-binding domains was more effective on the more hydrophilic 100%OH-SAMs. This fact, coupled to the ability of this type of SAMs to selectively recruit and retain VN in the presence of competitive serum proteins, seems to correlate with the better cell response observed on these Surfaces, as compared with hydrophobic O%OH(100%CH(3))-SAMs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10