Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Pedro P. Madeira (Autor) FEUP; José A. Teixeira (Autor) FEUP; Eugénia A. Macedo (Autor) FEUP; Larissa M. Mikheeva (Autor) FEUP; Boris Y. Zaslavsky (Autor) FEUP

Revista

Título: Fluid Phase Equilibria

Vol. 267

Páginas: 150-157

ISSN: 0378-3812

Editora: Elsevier

Indexação

ISI Web of Knowledge - 37 Citações

ISI Web of Science

Scopus - 38 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia química

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química ; Ciências Tecnológicas > Tecnologia

Outras Informações

ID Authenticus: P-003-ZBQ

DOI: 10.1016/j.fluid.2008.03.003

Abstract (EN): Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)-polyethylene glycol (PEG) and Dex-Ucon 50-HB-5100 (Ucon-a random copolymer of ethylene glycol and propylene glycol) both containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 degrees C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8