Liposome Delivery of Nucleic Acids in Bacteria: Toward In Vivo Labeling of Human Microbiota

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Moreira, L (Autor) Outra; Guimaraes, N (Autor) FEUP; Pereira, S (Autor) Outra; Santos, RS (Autor) Outra; Joana A Loureiro (Autor) FEUP; Maria do Carmo Pereira (Autor) FEUP; Azevedo, NF (Autor) FEUP

Revista

Título: Acs Infectious Diseases

Páginas: 1218-1230

ISSN: 2373-8227

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Outras Informações

ID Authenticus: P-00W-VGX

DOI: 10.1021/acsinfecdis.1c00601

Abstract (EN): Development of specific probes to study the in vivo spatial distribution of microorganisms is essential to understand the ecology of human microbiota. Herein, we assess the possibility of using liposomes loaded with fluorescently labeled nucleic acid mimics (LipoNAMs) to image Gram-negative and Gram-positive bacteria. We proved that liposome fusion efficiencies were similar in both Gram-negative and Gram-positive bacteria but that the efficiency was highly dependent on the lipid concentration. Notably, LipoNAMs were significantly more effective for the internalization of oligonucleotides in bacteria than the fixation/permeabilization methods commonly used in vitro. Furthermore, a structural and morphological assessment of the changes on bacteria allowed us to observe that liposomes increased the permeability of the cell envelope especially in Gram-negative bacteria. Considering the delivery efficiency and permeabilization effect, lipid concentrations of approximately 5 mM should be selected to maximize the detection of bacteria without compromising the bacterial cellular structure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13