Long-term functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Luis, AL (Autor) ICBAS; Amado, S (Autor) Outra; Geuna, S (Autor) Outra; Rodrigues, JM (Autor) FMUP; Simoes, MJ (Autor) Outra; Santos, JD (Autor) FEUP; Fregnan, F (Autor) Outra; Raimondo, S (Autor) Outra; Prieto P Veloso (Autor) Outra; Ferreira, AJA (Autor) Outra; Armada da Silva, PAS (Autor) Outra; Varejao, ASP (Autor) Outra; Mauricio, AC (Autor) ICBAS

Revista

Título: Journal of Neuroscience Methods

Vol. 163 Nº 1

Páginas: 92-104

ISSN: 0165-0270

Editora: Elsevier

Indexação

ISI Web of Knowledge - 93 Citações

ISI Web of Science

Scopus - 92 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia biomédica

Outras Informações

ID Authenticus: P-004-9BW

DOI: 10.1016/j.jneumeth.2007.02.017

Abstract (EN): We have recently described the sequence of functional and morphologic changes occurring after a standardized, sciatic nerve crush injury. An 8-week post-injury time was used because this end point is the far most used. Unexpectedly, both functional and morphological data revealed that animals had still not recovered to normal pre-injury levels. Therefore, the present study was designed in order to prolong the observation up to 12 weeks. Functional recovery was evaluated using sciatic functional index (SFI), static sciatic index (SSI), extensor postural thrust (EPT), withdrawal reflex latency (WRL) and ankle kinematics. In addition, quantitative morphology was carried out on regenerated nerve fibers. A full functional recovery was predicted by SFI/SSI, EPT and WRL but not all ankle kinematics parameters. Moreover, only two morphological parameters (myelin thickness/axon diameter ratio and fiber/axon diameter ratio) returned to normal values. Data presented in this paper provide a baseline for selecting the adequate end-point and methods of recovery assessment for a rat sciatic nerve crush study and suggest that the combined use of functional and morphological analysis should be recommended in this experimental model.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13