Development of Neuropeptide Y and Cell-Penetrating Peptide MAP Adsorbed onto Lipid Nanoparticle Surface

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Silva, S (Autor) Outra; Marto, J (Autor) Outra; Goncalves, LM (Autor) Outra; Fernandes, HS (Autor) Outra; Sergio Filipe Sousa (Autor) FCUP; Almeida, AJ (Autor) Outra; Nuno Vale (Autor) FMUP

Revista

Título: Molecules

Nº de Série 3 Vol. 27 Nº 969

Página Final: 2734

ISSN: 1420-3049

Editora: MDPI

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00W-EDA

DOI: 10.3390/molecules27092734

Abstract (EN): Functionalization of nanoparticles surfaces have been widely used to improve diagnostic and therapeutic biological outcome. Several methods can be applied to modify nanoparticle surface; however, in this article we focus toward a simple and less time-consuming method. We applied an adsorption method on already formulated nanostructured lipid carriers (NLC) to functionalize these nanoparticles with three distinct peptides sequences. We selected a cell-penetrating peptide (CPP), a lysine modified model amphipathic peptide (Lys(N-3)-MAP), CPP/drug complex, and the neuropeptide Y. The aim of this work is to evaluate the effect of several parameters such as peptide concentration, different types of NLC, different types of peptides, and incubation medium on the physicochemical proprieties of NLC and determine if adsorption occurs. The preliminary results from zeta potential analysis indicate some evidence that this method was successful in adsorbing three types of peptides onto NLC. Several non-covalent interactions appear to be involved in peptide adsorption with the possibility of three adsorption peptide hypothesis that may occur with NLC in solution. Moreover, and for the first time, in silico docking analysis demonstrated strong interaction between CPP MAP and NPY Y1 receptor with high score values when compared to standard antagonist and NPY.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17