The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Silva, C (Autor) FMUP; Andrade, N (Autor) Outra; guimaraes, jt (Autor) FMUP; Cardoso, E (Autor) FMUP; Meireles, C (Autor) Outra; Pinto, V (Autor) Outra; Paiva, J-A (Autor) FMUP; Martel, F (Autor) FMUP

Revista

Título: Cell Biochemistry and Function

Vol. 40

Páginas: 127-137

ISSN: 0263-6484

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00V-YK6

DOI: 10.1002/cbf.3681

Abstract (EN): Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12A(DMBA)) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, H-3-DG uptake and lactic acid production in both cell lines. The stimulatory effects of insulin upon cell proliferation and H-3-DG uptake were hampered by rapamycin, LY294001 and BAY-876, in both cell lines. In conclusion, hyperinsulinemia, one important characteristic of T2DM, contributes to the initiation of breast cancer by a PI3K- and mTOR-dependent mechanism involving increased GLUT1-mediated glucose uptake. Significance The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11