A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Mergulhao, FJM (Autor) Outra; Monteiro, GA (Autor) Outra; Cabral, JMS (Autor) Outra; Taipa, MA (Autor) Outra

Revista

Título: Molecular Biotechnology

Vol. 19

Páginas: 239-244

ISSN: 1073-6085

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 11 Citações

ISI Web of Science

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Tecnológicas > Tecnologia > Biotecnologia

Outras Informações

ID Authenticus: P-000-SYK

DOI: 10.1385/mb:19:3:239

Abstract (EN): A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit antibovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin to the growth medium of Escherichia coli cultures. The method here reported conjugates the advantages of sandwich-type ELISA assays, namely, high sensitivity, specificity, and throughput, with the possibility of quantifying small protein molecules (e.g., peptides). A further advantage of gene fusion techniques integrating both downstream processing and product detection and quantitation is highlighted. The method is capable of detecting levels of 0.05 ng of ZZ-proinsulin.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6