Evaluation of inducible promoters on the secretion of a ZZ-Proinsulin fusion protein

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Filipe Mergulhão (Autor) FEUP; Gabriel Monteiro (Autor) Outra; Gen Larsson (Autor) Outra; Maria Bostrom (Autor) Outra; Anne Farewell (Autor) Outra; Thomas Nystrom (Autor) Outra; Joaquim Cabral (Autor) Outra; Angela Taipa (Autor) Outra

Revista

Título: Biotechnology and Applied Biochemistry

Vol. 38 Nº Part 1

Páginas: 87-93

ISSN: 0885-4513

Editora: Wiley-Blackwell

Indexação

ISI Web of Science

Classificação Científica

CORDIS: Ciências Tecnológicas > Tecnologia > Biotecnologia

Outras Informações

DOI: 10.1042/BA20030043

Abstract (EN): Four inducible promoters, uspA, uspB, IacUV5 and malK, were evaluated in the expression of the fusion protein ZZ-proinsulin by Escherichia coli. The aim was to select for their effects on the most appropriate expression system (promoter and culture medium) for secretion of ZZ-proinsulin to the periplasmic space and culture medium. All the expression vectors contained the RNase III cleavage site to ensure that the mRNA translation rate remained independent of 5'-untranslated regions thus making promoter strength comparisons more accurate. The highest ZZ-proinsulin secretion yields were 6.2 mg/g of dry cell weight in the periplasmic space and 2.6 mg/g of dry cell weight in the culture medium using the malK promoter. It was also demonstrated that the use of M9 minimal medium favours secretion.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7