Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Pereira, AL (Autor) Outra; Pereira, AJ (Autor) Outra; Maia, ARR (Autor) Outra; Drabek, K (Autor) Outra; Sayas, CL (Autor) Outra; Hergert, PJ (Autor) Outra; Lince Faria, M (Autor) Outra; Matos, I (Autor) Outra; Duque, C (Autor) Outra; Stepanova, T (Autor) Outra; Rieder, CL (Autor) Outra; Earnshaw, WC (Autor) Outra; Galjart, N (Autor) Outra; Helder Maiato (Autor) FMUP

Revista

Título: Molecular Biology of the Cell

Vol. 17

Páginas: 4526-4542

ISSN: 1059-1524

Editora: American Society for Cell Biology

Indexação

ISI Web of Knowledge - 84 Citações

Scopus - 85 Citações

Outras Informações

ID Authenticus: P-004-GYC

DOI: 10.1091/mbc.e06-07-0579

Abstract (EN): CLASPs are widely conserved microtubule plus-end-tracking proteins with essential roles in the local regulation of microtubule dynamics. In yeast, Drosophila, and Xenopus, a single CLASP orthologue is present, which is required for mitotic spindle assembly by regulating microtubule dynamics at the kinetochore. In mammals, however, only CLASP1 has been directly implicated in cell division, despite the existence of a second paralogue, CLASP2, whose mitotic roles remain unknown. Here, we show that CLASP2 localization at kinetochores, centrosomes, and spindle throughout mitosis is remarkably similar to CLASP1, both showing fast microtubule-independent turnover rates. Strikingly, primary fibroblasts from Clasp2 knockout mice show numerous spindle and chromosome segregation defects that can be partially rescued by ectopic expression of Clasp1 or Clasp2. Moreover, chromosome segregation rates during anaphase A and B are slower in Clasp2 knockout cells, which is consistent with a role of CLASP2 in the regulation of kinetochore and spindle function. Noteworthy, cell viability/proliferation and spindle checkpoint function were not impaired in Clasp2 knockout cells, but the fidelity of mitosis was strongly compromised, leading to severe chromosomal instability in adult cells. Together, our data support that the partial redundancy of CLASPs during mitosis acts as a possible mechanism to prevent aneuploidy in mammals.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17