Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Wandke, C (Autor) Outra; Barisic, M (Autor) Outra; Sigl, R (Autor) Outra; Rauch, V (Autor) Outra; Wolf, F (Autor) Outra; Amaro, AC (Autor) Outra; Tan, CH (Autor) Outra; Pereira, AJ (Autor) Outra; Kutay, U (Autor) Outra; Helder Maiato (Autor) FMUP; Meraldi, P (Autor) Outra; Geley, S (Autor) Outra

Revista

Título: Journal of Cell Biology

Vol. 198

Páginas: 847-863

ISSN: 0021-9525

Editora: Rockefeller University Press

Indexação

ISI Web of Knowledge - 84 Citações

Scopus - 85 Citações

Outras Informações

ID Authenticus: P-002-5JZ

DOI: 10.1083/jcb.201110060

Abstract (EN): Chromokinesins are microtubule plus end directed motor proteins that bind to chromosome arms. In Xenopus egg cell-free extracts, Xkid and Xklp1 are essential for bipolar spindle formation but the functions of the human homologues, hKID (KIF22) and KIF4A, are poorly understood. By using RNAi-mediated protein knockdown in human cells, we find that only co-depletion delayed progression through mitosis in a Mad2-dependent manner. Depletion of hKID caused abnormal chromosome arm orientation, delayed chromosome congression, and sensitized cells to nocodazole. Knockdown of KIF4A increased the number and length of microtubules, altered kinetochore oscillations, and decreased kinetochore microtubule flux. These changes were associated with failures in establishing a tight metaphase plate and an increase in anaphase lagging chromosomes. Co-depletion of both chromokinesins aggravated chromosome attachment failures, which led to mitotic arrest. Thus, hKID and KIF4A contribute independently to the rapid and correct attachment of chromosomes by controlling the positioning of chromosome arms and the dynamics of microtubules, respectively.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17