Heterochromatin boundaries are hotspots for de novo kinetochore formation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Olszak, AM (Autor) Outra; van Essen, D (Autor) Outra; Pereira, AJ (Autor) Outra; Diehl, S (Autor) Outra; Manke, T (Autor) Outra; Helder Maiato (Autor) FMUP; Saccani, S (Autor) Outra; Heun, P (Autor) Outra

Revista

Título: Nature Cell Biology

Vol. 13

Páginas: 799-U166

ISSN: 1465-7392

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 99 Citações

Scopus - 96 Citações

Outras Informações

ID Authenticus: P-002-Q7N

DOI: 10.1038/ncb2272

Abstract (EN): The centromere-specific histone H3 variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse induction of CENH3 (Drosophila CID) in Schneider S2 cells leads to its incorporation into non-centromeric regions and generates CID islands that resist clearing from chromosome arms for multiple cell generations. We demonstrate that CID is lands represent functional ectopic kinetochores, which are non-randomly distributed on the chromosome and show a preferential localization near telomeres and pericentric heterochromatin in transcriptionally silent, intergenic chromatin domains. Although overexpression of heterochromatin protein1 (HP1) or increasing histone acetylation interferes with CID island formation on a global scale,induction of a locally defined region of synthetic heterochromatin by targeting HP1-LacI fusions to stably integrated Lac operator arrays produces a proximal hotspot for CID deposition. These data indicate that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and there by contribute to centromere identity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 19