Age-related changes in lipid peroxidation products in rat adrenal gland

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

Almeida, Henrique (Autor) FMUP; Magalhaes, MC (Autor) Outra; Magalhaes, MM (Autor) Outra

Revista

Título: Age

Vol. 21

Páginas: 119-121

ISSN: 0161-9152

Editora: Kluwer Academic Publishers

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-001-7G8

DOI: 10.1007/s11357-998-0018-4

Abstract (EN): Chloroform-methanol extracts from rat adrenals at five different ages (2, 6, 12, 18 and 24 months), were studied by fluorescence. After obtaining excitation and emission spectra, fluorescence intensity was measured at 365 nm excitation and 455 emission for all time points of aging. An additional study of lipid peroxidation employing a thiobarbituric acid reaction was made. Fluorescence intensity increased during aging from 16.39 x 10(3) arbitrary units of fluorescence per gram of tissue at 2 months, to 34.33 x 10(3) units at 24 months. Thiobarbituric acid reaction products expressed in nmol of malondialdehyde per gram of adrenal increased from 172.97 at 2 months to 640.83 at 24 months. One way analysis of variance revealed a statistically significant difference (p<0.05 and p<0.01 respectively). The results show an age-related steady increase in lipid peroxidation products in rat adrenals and suggest their accumulation in lipofuscin granules.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3