Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Barbosa, J (Autor) Outra; rodrigues, ag (Autor) FMUP; Perez, MJ (Autor) Outra; pina-vaz, c (Autor) FMUP

Revista

Título: Memorias do Instituto Oswaldo Cruz

Vol. 109

Páginas: 1078-1080

ISSN: 0074-0276

Editora: Fundação Oswaldo Cruz (Fiocruz)

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-00A-1B9

DOI: 10.1590/0074-0276140267

Abstract (EN): Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (105/mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3