Chromosome mis-segregation and cytokinesis failure in trisomic human cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Nicholson, JM (Autor) Outra; Macedo, JC (Autor) Outra; Mattingly, AJ (Autor) Outra; Wangsa, D (Autor) Outra; Camps, J (Autor) Outra; Lima, V (Autor) FMUP; Gomes, AM (Autor) Outra; doria, s (Autor) FMUP; Ried, T (Autor) Outra; Logarinho, E (Autor) Outra; Cimini, D (Autor) Outra

Revista

Título: eLife

Vol. 4

ISSN: 2050-084X

Editora: eLife Sciences Publications

Indexação

ISI Web of Knowledge - 80 Citações

Scopus - 76 Citações

Outras Informações

ID Authenticus: P-00G-B15

DOI: 10.7554/elife.05068

Abstract (EN): Cancer cells display aneuploid karyotypes and typically mis-segregate chromosomes at high rates, a phenotype referred to as chromosomal instability (CIN). To test the effects of aneuploidy on chromosome segregation and other mitotic phenotypes we used the colorectal cancer cell line DLD1 (2n = 46) and two variants with trisomy 7 or 13 (DLD1+7 and DLD1+13), as well as euploid and trisomy 13 amniocytes (AF and AF+13). We found that trisomic cells displayed higher rates of chromosome mis-segregation compared to their euploid counterparts. Furthermore, cells with trisomy 13 displayed a distinctive cytokinesis failure phenotype. We showed that up-regulation of SPG20 expression, brought about by trisomy 13 in DLD1+13 and AF+13 cells, is sufficient for the cytokinesis failure phenotype. Overall, our study shows that aneuploidy can induce chromosome mis-segregation. Moreover, we identified a trisomy 13-specific mitotic phenotype that is driven by up-regulation of a gene encoded on the aneuploid chromosome.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 23