Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Afonso, O (Autor) Outra; Castellani, CM (Autor) Outra; Cheeseman, LP (Autor) Outra; J Ferreira (Autor) FMUP; Orr, B (Autor) Outra; Ferreira, LT (Autor) Outra; Chambers, JJ (Autor) Outra; Morais de Sa, E (Autor) Outra; Maresca, TJ (Autor) Outra; Helder Maiato (Autor) FMUP

Revista

Título: eLife

Vol. 8

ISSN: 2050-084X

Editora: eLife Sciences Publications

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-00Q-ZAC

DOI: 10.7554/elife.47646

Abstract (EN): According to the prevailing 'clock' model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient - the 'ruler' model. Here we found that Cdk1 remains active during anaphase due to ongoing APC/C-cd(c2)0 - and APC/C-cdh1 -mediated degradation of B-type Cyclins in Drosophila and human cells. Failure to degrade B-type Cyclins during anaphase prevented mitotic exit in a Cdk1-dependent manner. Cyclin B1-Cdk1 localized at the spindle midzone in an Aurora B-dependent manner, with incompletely separated chromosomes showing the highest Cdk1 activity. Slowing down anaphase chromosome motion delayed Cyclin B1 degradation and mitotic exit in an Aurora B-dependent manner. Thus, a crosstalk between molecular 'rulers' and 'clocks' licenses mitotic exit only after proper chromosome separation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 31