Glial activation in the collagenase model of nociception associated with osteoarthritis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Adaes, S (Autor) Outra; Almeida, L (Autor) Outra; Potes, CS (Autor) Outra; Ferreira, AR (Autor) Outra; Castro-Lopes J.M. (Autor) FMUP; Ferreira-Gomes J. (Autor) FMUP; Neto F.L. (Autor) FMUP

Revista

Título: Molecular Pain

Vol. 13

ISSN: 1744-8069

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 21 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-00M-HFP

DOI: 10.1177/1744806916688219

Abstract (EN): Background: Experimental osteoarthritis entails neuropathic-like changes in dorsal root ganglia (DRG) neurons. Since glial activation has emerged as a key player in nociception, being reported in numerous models of neuropathic pain, we aimed at evaluating if glial cell activation may also occur in the DRG and spinal cord of rats with osteoarthritis induced by intra-articular injection of collagenase. Methods: Osteoarthritis was induced by two injections, separated by three days, of 500 U of type II collagenase into the knee joint of rats. Movement-induced nociception was evaluated by the Knee-Bend and CatWalk tests during the following six weeks. Glial fibrillary acidic protein (GFAP) expression in satellite glial cells of the DRG was assessed by immunofluorescence and Western Blot analysis; the pattern of GFAP and activating transcription factor-3 (ATF-3) expression was also compared through double immunofluorescence analysis. GFAP expression in astrocytes and IBA-1 expression in microglia of the L3-L5 spinal cord segments was assessed by immunohistochemistry and Western Blot analysis. The effect of the intrathecal administration of fluorocitrate, an inhibitor of glial activation, on movement-induced nociception was evaluated six weeks after the first collagenase injection. Results: GFAP expression in satellite glial cells of collagenase-injected animals was significantly increased six weeks after osteoarthritis induction. Double immunofluorescence showed GFAP upregulation in satellite glial cells surrounding ATF-3-positive neurons. In the spinal cord of collagenase-injected animals, an ipsilateral upregulation of GFAP and IBA-1 was also observed. The inhibition of glial activation with fluorocitrate decreased movement- and loading-induced nociception. Conclusion: Collagenase-induced knee osteoarthritis leads to the development of nociception associated with movement of the affected joint and to the activation of glial cells in both the DRG and the spinal cord. Inhibition of glial cell activation by fluorocitrate decreases these osteoarthritis-associated nociceptive behaviours. These results suggest that glial cell activation may play a role in the development of chronic pain in this experimental model of osteoarthritis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12