Cellulose phosphates as biomaterials. In vivo biocompatibility studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

J. C. Fricain (Autor) Outra; Pedro Lopes Granja (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP; B. de Jéso (Autor) Outra; N. Barthe (Autor) Outra; C. Baquey (Autor) Outra

Revista

Título: Biomaterials

Vol. 23 Nº 4

Páginas: 971-980

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 65 Citações

ISI Web of Science

Scopus - 73 Citações

COMPENDEX

Classificação Científica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais ; Ciências Tecnológicas > Engenharia > Engenharia biomédica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

Outras Informações

ID Authenticus: P-000-QBT

DOI: 10.1016/s0142-9612(01)00152-1

Abstract (EN): Femoral implantation of regenerated cellulose hydrogels revealed their biocompatibility, but a complete osseointegration could not be observed. Phosphorylation was therefore envisaged as the means to enhance cellulose bioactivity. In vitro studies showed that regenerated cellulose hydrogels promote bone cells attachment and proliferation but do not mineralize in acellular simulated physiological conditions. On the contrary, phosphorylated cellulose has shown an opposite behavior, by inducing the formation of a calcium phosphate layer in simulated physiological conditions, but behaving as a poor substrate for bone cells attachment and proliferation. In order to investigate the in vivo behavior of these materials, and assess the influence of mineralization induction ability vs. bone cells compatibility, unmodified and phosphorylated cellulose hydrogels were implanted in rabbits for a maximum period of 6 months and bone regeneration was investigated. Despite the difficulties arising from the retraction of cellulose hydrogels upon dehydration during the preparation of retrieved implants, histological observations showed no inflammatory response after implantation, with bone intra-spongious regeneration of cells and the integration of the unmodified as well as the phosphorylated cellulose implants. After a maximum implantation period of 6 months, histological observations, histomorphometry and the measurement of the amount of Ca-45 incorporated in the surrounding tissue indicated a slightly better osseointegration of phosphorylated cellulose, although no significant differences between the two materials were found.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10