Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Meireles, LA (Autor) Outra; Catarina Guedes, AC (Autor) Outra; F. Xavier Malcata (Autor) Outra

Revista

Título: Biotechnology and Bioengineering

Vol. 81

Páginas: 50-55

ISSN: 0006-3592

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 47 Citações

Outras Informações

ID Authenticus: P-000-J2B

DOI: 10.1002/bit.10451

Abstract (EN): The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain (tentatively called II#2) was obtained, the EPA and DHA contents of which (in % dry biomass) were 32.8% and 32.9% higher than those of the control, native strain. The final EPA yields, when the cultures were maintained under appropriate conditions, were 17.4 and 23.1 mg.g(-1) dry biomass, for the wild-type and the II#2 strain, respectively, whereas the final DHA yields were 8.0 and 10.6 mg.g(-1) dry biomass, respectively. These results suggest that random mutagenesis can successfully be applied to increase the yield of n-3 fatty acids by microalgae. (C) 2002 Wiley Periodicals, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6